By Angela F. Drew
In Atherosclerosis: Experimental equipment and Protocols, Angela Drew and a panel of specialists have assembled a accomplished number of conventional and state-of-the-art thoughts for investigating this ailment and its attainable remedies. each one easily reproducible approach contains step by step directions and sensible information about pattern assortment, the alternative of animal version method, experimental layout, and useful info research options. entire and richly exact, Atherosclerosis: Experimental tools and Protocols permits all biomedical investigators to pick these optimized options that could be such a lot fruitfully used to review the improvement, development, and therapy of atherosclerotic lesions at the present time.
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Additional info for Atherosclerosis: Experimental Methods and Protocols (Methods in Molecular Medicine)
Each UC method has Serum Analysis by Ultracentrifugation 29 differences in UC time, rotor speed, volume of sample required, and temperature at which the lipoprotein separation occurs, all of which may influence the result (7). Our laboratory (8) and others (7,9) have done numerous investigations establishing the optimal conditions for lipoprotein separation from plasma with minimal contamination or overlap between lipoprotein fractions. 21 g/mL. Furthermore, the makeup of these four fractions is elucidated; all lipid constituents and the total protein of each group are quantitated.
Drug Intervention The popular cholesterol-lowering drug probucol was fed to apoE-deficient mice to test the efficacy of the drug. Paradoxically, enhancement of atherosclerosis by probucol treatment was seen in apoE-deficient mice (26). References 1. Stary, H. , Blankenhorn, D. , Chandler, A. , Rosenfeld, M. , Schaffer, S. , Schwartz, C. , and Wagner, W. D. (1992) A definition of the intima of human arteries and of its atherosclerosis- prone regions. A report from the Committee on Vascular Lesions of the Council on Arteriosclerosis, American Heart Association.
They are synthesized by the intestine and are core-rich in TGs derived from dietary fat. VLDLs are the next largest lipoproteins (diameter 3–80 nm) and are also rich in TG. They are synthesized mainly by the liver, but may also be synthesized to a lesser degree by the intestine. IDLs, whose lipid core is comprised mainly of CE with some TGs, are the resultant product of VLDL metabolism. LDLs, in turn, are the product of IDL metabolism in which almost all of the remaining TGs have been hydrolyzed to produce a lipoprotein with a core comprised almost entirely of CE.